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Discriminant analysis applied to formants of vowels in Castellano dialect during the phonation with prosthesis and esophageal voice after total laryngectomy. The objective of the present work consists, in analyzing if there is statistically significant differences between certain quantitative variables of acoustic parameters of the voice, in three samples of individuals, using a discriminative analysis. The study groups are formed by: 20 patients with total laringectomy and esophageal voice, 20 patients with total laringectomy and tracheoesophageal voice with phonatory prosthesis, and 20 normal individuals. We analyzed the energy, bandwidth and frequency of the four first formants of the five Castilian vowels in the context of an established phrase. Based on our results the method of discriminative analysis of the used variables is valid to establish differences between the three studied groups; that is they display their own characteristics from the acoustic point of view. This method allows us to affirm that the tracheoesophageal voice does not come closer to the normal voice that the esophageal voice does.